English
SlovenčinaLaboratory of Cytoanalytics
contact info:
RNDr. Jana Jakubikova, PhD
jana.jakubikova@savba.sk
RNDr. Danka Cholujova, PhD
dana.cholujova@savba.sk
location:
Cancer Research Institute,
Biomedical Research Center SAS
Blok A, room# 5.29/5.30
Dubravska cesta 9
Bratislava 841 05, Slovakia
Phone: +421 2 3229 5158/5159
Infrastructure & Technology
BD FACSCanto II HTS
(analyzer -located 5.13)
Laser, Filter and Fluorochrome Options:
488nm Laser (20mW)
- (530nm) FITC
- (585nm) Pe
- (670nm) Pe Cy5
- (780nm) Pe Cy7
633nm Laser (15mW)
- (660nm) Apc
- (780nm) Apc Cy7
405nm Laser (60mW)
- (450nm) Pacific Blue
- (510nm) BV510
Accommodates:
*12x75mm tubes
*High Throughput Sampler (HTS) option
Processes 96 and 384 well microtiter plates
location:
Cancer Research Institute,
Biomedical Research Center SAS
Blok A, room# 5.13
Dubravska cesta 9
Bratislava 841 05, Slovakia
More information:
BD FACSAria II SORP UV
(sorter -located 5.14)
Laser, Filter and Fluorochrome Options:
488nm Laser (100mW)
- (530nm) FITC
- (575nm) Pe
- (685nm) PerCP
- (695nm) PerCP Cy5.5
- (780nm) Pe Cy7
640nm Laser (25mW)
- (670nm) Apc
- (730nm) Alexa 700
- (780nm) Apc-Cy7
405nm Laser (60mW)
- (450nm) Pacific Blue
- (525nm) BV510
- (670nm) BV650
355nm Laser
- (450nm) Live Dead Blue
- (530nm) Indo-1
Accommodates:
* 1.5ml Eppendorf tubes
* 12x75mm (5ml) tubes
* 15ml Conical tube
Cell conditions for sorting:
*Cell concentration: 15-20×106 per ml
*Cells must be FILTERED through a 70um filter (minimum)
NO Yeast Sorting
location:
Cancer Research Institute,
Biomedical Research Center SAS
Blok A, room# 5.14
Dubravska cesta 9
Bratislava 841 05, Slovakia
More information:
http://www.bdbiosciences.com/us/instruments/research/cell-sorters/bd-facsaria-iii/m/744763/overview
Resources:
Vendors
Beckman Coulter
www.beckmancoulter.com/wsrportal/wsr/index.htm
Becton Dickinson
www.bdbiosciences.com
Biolegend
www.biolegend.com/
Cell Signaling Technologies
www.cellsignal.com/
eBiosciences
www.ebioscience.com/
Invitrogen
www.invitrogen.com
Miltenyi
www.miltenyibiotec.com
Sony
http://www.sonybiotechnology.com
Software packages (Available to users of BMC Cytoanalytics Laboratory)
ModFit
http://www.vsh.com/products/mflt/index.asp
FCSExpress
www.denovosoftware.com
BD FACSDiva
www.bdbiosciences.com/instruments/software/facsdiva/index.jsp
Experimental design resources
Chromocyte
www.chromocyte.com/
Spectral viewers
BD Biosciences
www.bdbiosciences.com/research/multicolor/spectrum_viewer/index.jsp
eBiosiences
www.ebioscience.com/resources/fluorplan-spectra-viewer.htm
